Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice

Jianhui Cheng^1,2, Zuofa Zhang², Zhiguo Zheng^1,3, Guoying Lv², Liangyan Wang¹, Bing Tian¹, Yuejin Hua¹

¹Institute of Nuclear-Agricultural Sciences, Zhejiang University, Kaixuan Road No. 268, 310029 Hangzhou; ²Department of Horticulture, Zhejiang Academy of Agricultural Sciences, Shiqiao Road No. 139, 310021 Hangzhou; ³Cancer Research Institute, Zhejiang Cancer Hospital, Guangji Road No. 139, 310020 Hangzhou, China.

For correspondence:- Yuejin Hua Email: yjhua@zju.edu.cn Tel:+8657186971703

Received: 18 October 2013 Accepted: 6 February 2014 Published: 23 April 2014

Citation: Cheng J, Zhang Z, Zheng Z, Lv G, Wang L, Tian B, et al. Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice. Trop J Pharm Res 2014; 13(4):573-580 doi: 10.4314/tjpr.v13i4.13

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the antioxidant activity and hepatoprotective effect of deinoxanthin-rich extract from Deinococcus radiodurans (EDR) against CCl₄-induced liver injury in mice.

Methods: The ethanol extract of EDR was analyzed by liquid chromatography/mass spectrometry (LC/MS), and its antioxidant activity was examined using in vitro assays for reducing power, iron chelating activity and lipid peroxidation. The extract was also evaluated for its hepatoprotective activity against carbon tetrachloride-induced liver injury in mice. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) in serum, and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissue, as well as hepatic malondialdehyde (MDA) levels, were measured to monitor liver injury. Damage to liver cells was assessed by histology.

Results: EDR displayed strong reducing activity and lipid peroxidation inhibition activity in vitro. Pretreatment with EDR (400 mg/kg b.w.) significantly reduced activities of serum ALT, AST and ALP, as well as hepatic MDA levels (p < 0.05), but increased the activities of GSH-Px, CAT and SOD. Histopathological assessment showed that liver tissue damage was decreased by the protective effect of EDR.

Conclusion: The results show that EDR can protect mice against CCl₄-induced hepatic damage by its antioxidant and free radical scavenging activities.

Keywords: Antioxidant, Deinoxanthin, Deinococcus radiodurans, Hepatoprotective, Carbon tetrachloride, Liver damage